Composite

Part:BBa_K3440009:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


bphR2-Myc under Pconst


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 320


Design Notes

This part was designed to show that bphR2 can be expressed under Pconst. A myc tag was added for easier characterization. A moderate strength RBS (BBa_B0034) was used.

Source

bphR2 from Pseudomonas oleovorans

References